JCS -- Sridharan et al. 116 (5): 823 Figure IG7

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Fig. 7. Binding of ${alpha}$ SpII ${Sigma}$ *, FANCA and XPF to each other in the nucleus. Chromatin-associated proteins extracts from normal human lymphoblastoid cells were immunoprecipitated with anti- ${alpha}$ -spectrin, anti-FANCA and anti-XPF antibodies. (A) Immunoprecipitation was carried out using anti- ${alpha}$ -spectrin antibody or mouse IgG₁ (as a control). (B) Proteins were immunoprecipitated using anti-FANCA antibody or rabbit serum. (C) Immunoprecipitation was done using anti-XPF antibody and mouse serum. In all of the above immunoprecipitations (A-C), immunoblot analysis was carried out using anti- ${alpha}$ SpII ${Sigma}$ * (top panel), anti-FANCA (second panel) or anti-XPF (third panel).