Fig. 1. Toc34 and mutants. Toc34, (2-230)Toc34, Toc34C++ and (2-230)Toc34C++ are shown, indicating the deletions and the charge distribution of the 15 amino acids flanking the transmembrane domain. The amino acid sequence of the short constructs is given and the exchanged amino acids are presented in bold. The position of the [³⁵S]-labelled methionines and [³H]-labelled leucines is also shown.