Fig. 6. Insertion of Toc34 and variants into protein-free liposomes. [³H]-Leucine-labelled Toc34 (upper, left), (2-230)Toc34 (lower, left), Toc34C++ (upper, right) and (2-230)Toc34C++ (lower, right) were incubated with liposomes (1 mM final lipid concentration, composition C3), and binding and insertion was quantified as described in the legend for Fig. 5. The binding of Toc34 to protein-free liposomes was set to 100%. The results represent an average of at least three independent experiments and the numbers in brackets indicate the s.e.m. The models indicate the orientation of the proteins, and C the location of the C-terminus. (B) Insertion of [³H]-leucine-labelled (lanes 1-3) and [³⁵S]-methionine-labelled (lanes 4-6) (2-230)Toc34 (upper panel) and (2-230)Toc34C++ (lower panel) into liposomes (lanes 2, 3, 5 and 6) was performed as described in Fig. 2 and followed by thermolysin treatment (Thr, lanes 3 and 6). In lanes 1 and 4, 10% of the translation product is shown.