Fig. 7. Insertion of Toc34 and variants into protein-free liposomes lacking PG. (A) Insertion of [³H]-leucine-labelled Toc34 (upper, left), (2-230)Toc34 (lower, left), Toc34C++ (upper, right) and (2-230)Toc34C++ (lower, right) into liposomes of the composition C5 (Table 1) was performed, quantified and presented as described in the legend for Fig. 6. (B) Insertion of [³H]-leucine-labelled (lanes 1-3) and [³⁵S]-methionine-labelled (lanes 4-6) (2-230)Toc34 (upper panel) and (2-230)Toc34C++ (lower panel) into protein-free liposomes not containing PG (lanes 2, 3, 5 and 6) was performed as described in Fig. 2 and followed by thermolysin treatment (Thr, lanes 3 and 6). In lanes 1 and 4, 10% of the translation product is shown.