Fig. 5. Migration of MDCK cells transfected with dsRed-clathrin. Cells were imaged via time-lapse epifluorescence microscopy migrating towards the area of monolayer wounding (the top of the field). This figure presents the first (A) and last (B) images of a 1 hour time-lapse sequence, and an overlay (C) of cell borders drawn to illustrate net cell displacement. Scale bar, 20 µm.