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Fig. 2. Sterol localisation correlates with sites of active cell growth and cytokinesis. Sterol localisation detected by filipin staining in (A) nitrogen-starved cells, (B) cells released from nitrogen starvation for 80 minutes, (C) cdc10-V50 cells at 36°C, (E) glucose-starved cells, (F) cells grown in medium with glucose, (G) cdc25-22 cells at 36°C, (H) orb1-13 cells at 36°C, (I) a branched TBZ-treated cdc10-V50 cell, (J) mating cells, (K) ascospores and (L) mid1-18 cells at 36°C. (D) Localisation of CHD-GFP in cdc10-V50 cells.