Fig. 5. Structural alterations of sterol-rich membrane domains affect cytokinesis in S. pombe. (A,B) cdc4GFP cells were grown for 1 hour in medium containing 5 µg/ml filipin or 0.1% (v/v) DMSO as solvent control. Arrow indicates mis-shapen Cdc4-GFP ring; arrowhead indicates Cdc4-GFP spots. (C) cdc4GFP cells overexpressing C-4 sterol methyl oxidase under the nmt1-promotor. (D,E) Quantitation of localisation patterns. Bars in D represent filipin-treated cells (grey) and DMSO-treated cells (white); bars in E represent cells overexpressing C-4 sterol methyl oxidase (dark grey), control cells with empty vector (light grey) and control cells in 15 µM thiamine (white).