Fig. 6. Structural alterations of sterol-rich membrane domains compromise the stability of a colocalising plasma membrane protein. (A,B) bgs4GFP cells were grown for 1 hour in medium containing 5 µg/ml filipin or 0.1% (v/v) DMSO as solvent control. (C) Equal amounts (11.5 µg each) of total protein from filipin- or DMSO-treated cells were analysed by immunoblotting and probed with anti-GFP antibodies. The signal strength was quantified and plotted as relative units.