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Fig. 2. Subcellular localization of ß-catenin and p120 catenin in Cx43 CKO hearts. (A) Immunoblot analysis of cytosol fractions from control and CKO mouse hearts for the presence of ß-catenin and p120 catenin. (B) Immunoblot analysis of neat plasma membrane fractions from control and CKO mouse hearts for the presence of ß-catenin and p120 catenin. Coomassie staining was used to ensure equivalent loading of protein per lane in the cytosol and plasma membrane blots. (C) Immunoprecipitation of plasma membrane fractions with an anti-pan-cadherin antibody followed by blotting with an anti-p120 catenin antibody demonstrates an association of cadherin with p120 in control and CKO hearts.