Fig. 3. Immunofluorescent staining for cadherin in control and Cx43 CKO mouse heart sections. Heart sections were double stained with a mouse anti-pan-cadherin antibody (A, control; D, CKO), and a rabbit anti-Cx43 antibody (B, control; E, CKO) and imaged with a confocal microscope. Merged images are shown in C (control) and F (CKO) and demonstrate that adherens junction localization to the intercalated disc, indicated by cadherin staining, is unchanged in the absence of Cx43. Magnification, 40x; bar, 50 µm.