Fig. 3. Expression of mouse Pins in the developing nervous system. (A-C) Expression in the E12.5 embryonic CNS. Pins in situ hybridization on sagittal sections of an E12.5 embryo at the trunk level (A) and at the level of the midbrain and forebrain (B) shows expression restricted to the ventricular zone of the CNS. (C-C'') Transversal sections at the level of the right ventricle of the forebrain: Pins expression domain (C) corresponds to the proliferating cells of the ventricular zone, as revealed on an adjacent section by an anti Ki-67 staining (C'). (C'') Hoechst staining of all nuclei in the section seen in C'. Anterior is up. (D-E) Pins expression in the PNS at E12.5: dorsal root ganglia, seen on a parasagittal section (D), cranial sensory ganglia and sympathetic ganglia, seen on a transversal section (E), express Pins at E12.5. (E') On a section adjacent to E, the cranial sensory complex (IX-X) and the superior cervical ganglion of the sympathetic chain (stronger signal) are visualized with an anti-Phox2a antibody (Tiveron et al., 1996). (F) Expression in the E15.5 brain: Pins is detected along the ventricular zone, with most expression seen in the forebrain. Some expression is also observed in the roof of the diencephalon (arrow) at the ventricular level. See G for a higher magnification of the boxed region. (G-G'') Pins expression is restricted to the proliferating zone: (G) Pins, (G') Ki-67 in an adjacent section, (G'') Hoechst staining of the nuclei in the section seen in G'. (H-H'') higher magnification of sections shown in G-G'', as boxed in G''.