Fig. 1. TES is a component of cell adhesions. (A) Schematic representation of TES constructs. The deletion constructs used in these studies are demonstrated. Full-length TES contained amino acids 1-421. LIM-only TES, pEGFP-C1 contained amino acids 231-421 and pAS2-1 and pACT2 contained amino acids 228-421. LIM-less TES, pEGFP-C1 contained amino acids 1-246 and pGEX-2T contained amino acids 1-233. LIM 1 contained amino acids 230-297. LIM 1 and 2 contained amino acids 230-360. LIM three contained amino acids 351-421. (B) Localisation of full-length TES in Rat-1 fibroblasts. pEGFP-C1 vector (i) or full-length GFP-tagged TES (ii-ix) were stably expressed in Rat-1 fibroblasts as described in Materials and Methods. Cells were stained with anti-paxillin antibody and TRITC-labelled mouse secondary antibody to visualise endogenous paxillin (viii and ix). Solid arrows point to focal adhesion structures, dotted arrows to areas of cell-cell contact, and arrowheads to actin stress fibres. Inset is enlarged region. Bar, 10 µm.