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Fig. 2. Localisation of LIM-less and LIM-only regions of TES. Rat-1 fibroblasts stably expressing GFP-tagged LIM-only TES (A-F) or GFP-tagged LIM-less TES (G-I). Cells were stained with anti-paxillin antibody and TRITC-labelled mouse secondary antibody to visualise endogenous paxillin (E,F,N,O) or TRITC-labelled phalloidin to visualise actin stress fibres (K,L). Solid arrows point to focal adhesions, dotted arrows to areas of cell-cell contact and arrowheads to actin stress fibres. Bar, 10 µm.