Fig. 5. Pav-KLP becomes focused at the apexes of cones. Pav-KLP (red), -tubulin (green), and DNA (blue) in wild-type (A and B) and dd4^S (C and D) meiocytes. (A) Wild-type cyst in prometaphase/metaphase revealing ring canals derived from premeiotic divisions containing Pav-KLP (arrow). (B) Meiocytes in telophase II showing contracting rings with Pav-KLP staining around the central-spindle mid-zone (arrow). (C) A dd4^S cyst with Pav-KLP-containing ring canals (large arrow). This cysts also contains two hemi-spindles with Pav-KLP staining at the putative plus ends of microtubules at the periphery of the asters (arrowheads). A cone is also indicated by the small arrow in with Pav-KLP accumulated at its apex. (D) dd4^S meiocytes with Pav-KLP at the constriction point of cones (arrowheads), and at the apex of two biconical structures (arrows). Bar, 50 µm.