Fig. 2. Migration and net-like formation. (A) The number of migrated cells counted from 4 fields each on duplicate membranes (see Materials and Methods). (B) Number of net-like structures in monoculture (M) and co-culture (C). (C,D) Images of monoculture and co-culture, used for quantification (magnification x100). (E,F) Morphology of HUVECs in monoculture and co-culture respectively (magnification x400). (G) The appearance of net-like structures is abrogated by anti-angiogenic agents. The figure shows average numbers of enclosed spaces per field for HUVEC monoculture (M), HUVECs in co-culture (C) and HUVECs in co-culture with added angiostatin (C/angio) or endostatin (C/endo) at 100 ng ml^–1. Error bars are standard deviations.