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Fig. 1. Molecular map of the pros54 genomic region. (A) Position of the pros54, Vha M9.7-2 and CG7181 genes according to GadFly. Arrows show the location and the direction of these genes. The triangle labels the site of P-lacW0554/18 insertion. The E and PIR PCR primers used to screen for genomic deletions are indicated in the upper part of the figure. S, E and P are SacI, EcoRI and PstI restriction sites, respectively. Numbers in parentheses give the nucleotide-scale positions according to GadFly. (B) Extension of Df(3L)pros54P(w+). (C) Restriction fragments used in rescue experiments (see text).