Fig. 5. Developmental profile of the 26S proteasome in the Oregon R wild-type strain. Total protein extract from 0- to 24-hour-old embryos (lane 1), 3^rd instar larvae (lane 2), 0-hour-old (lane 3), 2-hour-old (lane 4), 4-hour-old (lane 5), 6-hour-old (lane 6), 8-hour-old (lane 7), 10-hour-old (lane 8) prepupae as well as 12-hour-old (lane 9), 18-hour-old (lane 10) and 24-hour-old (lane 11) pupae were fractionated on 8% SDS-PAGE and immunoblotted with a mixture of two different monoclonal antibodies specific for subunits p54 and p48A. The age of the specimens is given in hours after white puparium formation.