JCS -- Goriounov et al. 116 (6): 1045 Figure IG2

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Fig. 2. Localization of the ${alpha}$ isoforms of hGAR17 and hGAR22 in transfected cells. NIH 3T3 (A-F) or COS7 (G-L) cells were transiently transfected with pFLAG-hGAR17 ${alpha}$ A-C; G-I) or pFLAG-hGAR22 ${alpha}$ (D-F; J-L) and stained with a monoclonal anti-FLAG antibody (A,D,G,J), phalloidin-AlexaFluor-594 (B,E) and a polyclonal anti-tubulin antibody (H,K). (C,F,I,L) Superimposed images, with the FLAG staining in green and the phalloidin or tubulin staining in red. Both ${alpha}$ proteins colocalized with MFs (C,F) but not with MTs (I,L). Similar results were obtained with untagged proteins. Bar, 15 µm.