Fig. 3. Localization of the ß isoforms of hGAR17 and hGAR22 in transfected
cells. NIH 3T3 (A-F) or COS7 (G-L) cells were transiently transfected with
pFLAG-hGAR17ß (A-C; G-I) or pFLAG-hGAR22ß (D-F; J-L) and stained
with a monoclonal anti-FLAG antibody (A,D,G,J), phalloidin-AlexaFluor-594
(B,E) and a polyclonal anti-tubulin antibody (H,K). (C,F,I,L) Superimposed
images, with the FLAG staining in green and the phalloidin or tubulin staining
in red. Both ß proteins exhibited colocalization with MFs (C,F) and MTs
(I,L; see insets). Bar, 15 µm.