Fig. 5. Localization of C-terminal fragments of hGAR17 and hGAR22 in transfected cells. COS7 cells were transiently transfected with pFLAG-hCt17 (A-C), pFLAG-hCt22 (D-F), pFLAG-hGARt17 (G-I) or pFLAG-hGARt22 (J-L) and stained with a monoclonal anti-FLAG antibody (A,D,G,J) and a polyclonal anti-tubulin antibody (B,E,H,K). Superimposed images, with the FLAG staining in green and the tubulin staining in red, are shown in panels C, F, I and L. Both Ct proteins accumulated in the nucleus (A,D), whereas the cytoplasmic protein showed some colocalization with MTs (C,F; see insets). The GARt fragments colocalized with and appeared to bundle MTs (I,L). Bar, 15 µm.