Fig. 10. Visualization of A-crystallin and ß-tubulin in lens epithelial cells during mitosis. Wild-type mouse lens epithelial cells were fixed and stained with anti-A and an Alexa⁴⁸⁸-conjugated secondary antibody (green). Cells were also immunostained with an antibody to ß-tubulin and an Alexa⁵⁶⁸-conjugated secondary antibody (red) and the nuclei were stained with TOPRO-3 (blue). (A-C) Metaphase, (D-F) anaphase and (GI) cytokinesis. Note that A was concentrated in the region of the centrosome in metaphase and in the intercellular bridge microtubules of the dividing cells during cytokinesis (arrowheads). Bars, 10 µm (A-F); 25 µm (G-I).