Fig. 5. Comparison of myosin 1C (green) and myosin IIA (red) staining in wt and KO growth cones (cold methanol fixed). (A) A KO growth cone from a SCG neuron. The myosin IC staining is mostly confined to the neurite, whereas the myosin IIA staining is distributed throughout the growth cone. Wt SCG growth cones showed a similar pattern, although the growth cones were usually larger. (B) A wt DRG growth cone shows a similar pattern of staining compared to that seen in SCG neurons (a relatively small cone was selected for comparison purposes). The intensity of the myosin IC staining appears to be elevated in the neurite compared with that observed in SCG neurons. This was consistently observed, but it is unclear if this results from thickness differences in the two types of neurites. (C) A KO DRG growth cone also shows a similar pattern and intensity of staining to the wt DRG growth cone. C, bar, 6.8 µm.