Fig. 6. Western blot analysis of CDP II in different subcellular fractions. Cell-free extract (300 g supernatant, 180 µg), nuclear (154 µg), mitochondrial (145 µg), plasma membrane (165 µg) and soluble (185 µg) protein fractions were electrophoresed in 11% SDS-PAGE and immunoblotted as described in Materials and Methods. Lanes: (1) cell-free extract; (2) nuclear; (3) mitochondrial; (4) plasma membrane; (5) soluble protein fraction.