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Fig. 5. Proteolytic sensitivity of Vph1p depends on Vtc proteins. (A) 25 µg of vacuoles from the indicated strains expressing Vph1p with chromosomally encoded C-terminal tags were precipitated with TCA and analyzed by SDS-PAGE and western blotting with the indicated antibodies. All strains were derived from OMY1, that is, deficient for vacuolar proteases ({Delta}pep4, {Delta}prb1). (B) Same analysis as in A, but with whole cell extracts from 107 cells.