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Fig. 6. Levels of V-ATPase subunits in different deletion mutants. (A) 30 µg each of vacuoles from the indicated strains were precipitated with TCA and analyzed by SDS-PAGE and western blotting with the indicated antibodies. All strains were deficient for vacuolar proteases ({Delta}pep4). Strain backgrounds were OMY1 (lanes 1-4) and SBY86 (lanes 5-6). (B) Same as in A, but with whole cell extracts.