Fig. 3. Proliferation and apoptosis in MECs of ß-eng transgenic mice. Immunofluorescent detection of BrdU incorporation in mammary epithelium of wild-type (A) and transgenic (B) mice revealed decreased proliferation in ß-eng transgenics (C). Fluorescent TUNEL assay revealed increased apoptosis in MECs from transgenic (E) compared with wild type (D). BrdU and TUNEL signals are represented in green. Nuclei are visualized by DAPI stain (blue). Bars, 100 µm. *P<0.001.