Fig. 10. INCENP labelling at telophase II poles and round spermatids (INCENP, green; ACA serum, red; DAPI, blue). (A-C) Partial projection of two focal planes throughout an endon view of an early telophase II pole. INCENP signals colocalize with kinetochores (arrows). (D-F) Partial projection of two focal planes throughout an end-on view of a late telophase II pole. INCENP signals appear as diffuse and faintly labelled clouds surrounding kinetochores. (G,H) Single plane of an early round spermatid. INCENP localizes at a centrally located and large brightly labelled chromocentre containing centromeres. (I,J) Single plane of a late round spermatid where only centromere signals are observed. Bar, 5 µm.