Fig. 7. INCENP distribution in metaphase I spermatocytes (INCENP, green; ACA serum, red in B, C, E, F, H, I, K and L; SCP3, red in N and O; DAPI, blue). (A-F) Two partial projections, each one from three focal planes, throughout the same metaphase I spermatocyte. INCENP labels the centromeres, as detected with the ACA serum. A diffuse faint labelling is observed on bivalents, and a bright signal is discerned at the interchromatid domain (arrowheads) in the sex (XY) bivalent. (G-L) Two selected metaphase I autosomal bivalents. INCENP labels a T-shaped structure beneath the kinetochore labelling obtained with the ACA serum, although both signals partially colocalize. (M-O) Selected metaphase I centromere. INCENP and SCP3 colocalize at a T-shaped structure. (A-F) Bar in F, 10 µm. (G-L) Bar in L, 2 µm. (M-O) Bar in O, 0.5 µm.