Fig. 1. Double immunostaining of serum-starved SV80 cells for vinculin (left column) and phosphotyrosine (middle column) following treatment with nocodazole for up to 60 minutes. The right column shows the ratio between vinculin and PY intensities. All images are presented in a spectrum scale as indicated in the lookup table for each column. Although focal complexes are most prominent at time=0, FAs are apparent at 3 minutes, and constitute the majority of adhesive structures from 10 minutes onwards. FAs reach their maximal size at 30 minutes. Vinculin intensity increases from 0 to 30 minutes. Note the `centripetal shift' of vinculin relative to PY at 0 and 3 minutes, which can still be seen in some FAs after 10 minutes (arrowheads). Bar, 1 µm.