Fig. 2. Double immunostaining of paxillin and phosphotyrosine. All images were acquired and produced as in Fig. 1. Whereas paxillin levels are continuously increasing from 0 to 30 minutes, PY levels increase significantly only later, between 3 and 10 minutes, and then reach a plateau. Note the overall extensive overlap between paxillin and PY labeling. Main differences are detected along the paxillin-rich and PY-poor peripheral margins of FAs at 30 minutes (arrowheads). Bar, 1 µm.