Fig. 4. Quantitative analysis of the immunostaining data shown in Figs 1,2,3, depicting the percent increase in average intensity and area compared with control, untreated cells. Vinculin, paxillin and FAK levels are already elevated after 3 minutes of treatment, whereas PY levels remain essentially unchanged. Vinculin and paxillin levels continue to increase until 30 minutes and decrease only slightly afterwards, whereas FAK and PY levels reach a plateau after 10 minutes and decrease to control levels between 30 and 60 minutes. FA growth starts immediately and continues for 30 minutes.