Fig. 7. Frames from a two-color movie showing a serum-starved SV80-cell transfected with CFP-vinculin and YFP-dSH2 and treated with nocodazole. Row 1 shows the CFP-vinculin staining at 4 different time points, and the images in row 2 are time ratio (FRIT) images comparing consecutive time points. Row 3 and 4 show the corresponding YFP-dSH2 images. Row 5 shows the vin/dSH2 ratio at all time points. Note the strong initial increase in vinculin after 2 minutes, in comparison with the low and inconsistent changes in PY levels. The adhesions used for the quantification presented in Fig. 8 are marked in the 0' frame of YFP-dSH2. The whole movie (2 colors and ratio) can be found in the online supplemental material (http://jcs.biologists.org/supplemental). Bar, 10 µm.