Fig. 8. Quantitative analysis of the three FAs marked in Fig. 7. The temporal changes of the integrated intensities (total label present in each adhesion) of CFP-vinculin and YFP-dSH2 are presented. Note that vinculin intensity increases sharply during the first two minutes in all three cases, whereas there is almost no increase in PY at the same time. The fourth panel shows the average values of all FA-associated CFP-vinculin and YFP-dSH2 in the tested cell, which is consistent with the data obtained for the fixed cells (see Fig. 4).