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Fig. 3. Microtubules, microfilaments and intermediate filaments within the cytoskeleton of endothelial cells visualized with GFP-tubulin, rhodaminated-phalloidin and antibodies to vimentin, respectively. (A) Microtubules (green) span large regions of the cytoplasm and often appear curved in form. (B) Microfilaments (green-yellow) appear linear in form within long stress fibers and triangulated actin `geodomes'; blue staining indicates nuclei. (C) Intermediate filaments (red) appear within a spread cell as a reticulated network that extends from the nucleus to the cell periphery.