Fig. 4. dy, Min and dyl are expressed in cuticle-forming tissues. dy (A,D) and dyl (C,F) RNA in situ hybridisation and anti-Min immunostaining (B,E) in wild-type embryos of stage 16-17. (A-C) A view of the embryonic epidermis. By stage 16, the three genes are upregulated in regions corresponding to the dorsal and ventral denticle belts (arrowheads). (D-F) View of embryonic internal structures. dy and Min are expressed in the pharynx (ph), oesophagus (es), the duct of the salivary glands (sgd) and in the hindgut (hg). dyl is present in the epidermis but also in the pharynx and oesophagus (arrowheads in F). (G-I) Pupal wings dissected at 28 hours APF and stained as above. At this stage all wing cells express both dy (G) and Min (H), but not dyl (I).