Fig. 2. Fluorescence micrographs of hippocampal neurons, labeled with BODIPY-ceramide for 30 minutes at room temperature and chased for 2.5-3 hours at 37°C. The images were recorded in the red channel, which reveals only highly concentrated label. Intense labeling of the Golgi complex and of vesicle clusters in neurites and growth cones is evident. The growth cones of these neurons are shown in the time series in Fig. 4.