Fig. 2. Overexpression of p0071 wt in MCF-7 cells. P0071 wt with a C-terminal DsRed tag or an N-terminal EGFP tag was expressed in MCF-7 cells. Cells were fixed in methanol and labeled with desmoplakin (serum 616) or E-cadherin antibodies. Plasma membrane regions denoted by arrows are shown in detail at higher magnification. Note that desmoplakin staining disappears from the membrane regions enriched in p0071, whereas E-cadherin staining is increased. Bars, 3 µm.