Fig. 1. The cflB gene is localized to the plasma and internal membranes, and co-localizes with actin. The gpdA(p)gfp::cflB strains were grown for 2 days at 25°C. CflB visualized in live cells (A,B) and in fixed cells by immunocytochemistry (C,D). (A) Apical hyphal cells. The GFP::CflB fusion protein is not present in large amounts at the hyphal apex. (B) Subapical hyphal cells. The GFP::CflB fusion protein is localized at the plasma membrane, at internal membranes and at septation sites. The single white arrows indicate the GFP::CflB fusion protein localized at septa. (C) CflB co-localizes with actin at the hyphal apex. Actin is concentrated at sites of polarized growth, such as the hyphal apex, indicated by the white arrow (anti-actin). The GFP::CflB fusion protein co-localizes with actin at the hyphal apex, shown by the white arrow (anti-GFP). (D) CflB co-localizes with actin at nascent septation sites. Actin is localized at nascent septation sites, indicated by white arrows (anti-actin). The white arrows under anti-GFP and CAL staining indicate the GFP::CflB fusion protein, which also localizes at nascent septation sites, and the corresponding cell walls, respectively. Scale bars, 20 µm.