Fig. 5. The cflB gene affects asexual development in A. nidulans. (A) Colonial morphology of A. nidulans cflB strains. Strains were grown for 2 days at 25°C on noninducing (nonind.) or inducing (induced) medium. The alcA(p)::cflB, alcA(p)::cflB^D123A dominant-negative and alcA(p)::cflB^G18V dominant-activated transformants displayed a decrease in conidiation under inducing conditions that was more severe in the transformants with dominant alleles. (B) Strains were induced during conidiation by growth for 1 day at 25°C and induction for an additional day. The arrowheads indicate conidiophore structures (c, conidia; m, metulae; p, phialides; v, vesicle). The alcA(p)::cflB⁺, alcA(p)::cflB^D123A and alcA(p)::cflB^G18V transformants showed conidiophores with inappropriate polarized growth where metulae and phialides could be greatly extended. Scale bars, 20 µm. Images were captured using DIC or DAPI (to observe nuclei).