Fig. 8. The cflB gene is not required for correct actin localization in arthroconidiating hyphae and in yeast cells. Wild-type and cflB strains were grown for 4 days at 37°C. Actin localization was examined using immunocytochemistry. (A) Colonial morphology at 37°C. Colonies of the cflB strain are reduced in size and possess irregular edges. (B) Arthroconidiating hyphae are shown at 37°C prior to cell separation. The cflB strain possesses swollen arthroconidiating hyphae that are highly septate. The white arrows indicate concentrated actin in both wild-type and cflB strains. (C) Yeast cells at 37°C. The single white arrow indicates actin concentrated in a yeast cell of both the wildtype and the cflB strain. (D) Nascent septation sites in vegetative hyphae at 37°C. Two regions of concentrated actin are indicated by single white arrows in the FITC panel. The corresponding cell wall is indicated by a single arrow in the CAL panel. Double arrows indicate completed double septation sites. (E) Magnification of the actin staining regions marked in D, where the arrows indicate the two regions of concentrated actin. Scale bars, 20 µm.