Fig. 2. Time course of IP₃R1 redistribution. The percentage of cells with IP₃R1 located in the perinculear region (squares) and in the cytoplasm (circles) after addition of 3 µM AVP is depicted for different time periods. The dashed line represents the removal of AVP (3 µM) after 2 hours, after which the cells where incubated in PBS without Ca²⁺ for the indicated time periods. Each result is the mean±s.e.m. from three independent experiments. The s.e.m. is not indicated when smaller than the symbol.