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Fig. 4. Visualization of the microtubular and actin cytoskeleton. Cells were fixed, permeabilized and incubated (A,C,E) with anti-{alpha} tubulin (1/2000) or (B,D,F) with rhodamine-phalloidin (1 unit/ml). (A,C,E) Anti-mouse FITC (1/400) was used as secondary antibody. The microtubular and actin cytoskeleton was visualized (A,B) before and after (C,D) AVP (3 µM) or (E,F) OAG (50 µM) treatment for 5 hours. Bar, 10 µm.