Fig. 6. Endoplasmic reticulum structure of A7r5 cells. ER structure was visualized in cells transfected with the pEYFP-ER vector, which encodes an ER-targeted yellow fluorescent fusion protein. The general ER morphology of (A) unstimulated and (B) AVP (3 µM, 5 hours) stimulated cells did not show significant differences. PDI was visualized (C) before and (D) after stimulation with AVP (3 µM, 2 hours) using the monoclonal anti-PDI antibody (1/100) and anti-mouse FITC (1/750). Bar, 5 µm.