Fig. 7. Visualization of SERCA pumps in A7r5. Cells were fixed, permeabilized and incubated with AS809-27 polyclonal antibody (1/300). Anti-rabbit FITC (1/750) was used as secondary antibody. SERCA was visualized in (A) resting cells and (B) after stimulation with AVP (3 µM, 2 hours). Bar, 10 µm.