Fig. 2. Analysis of the expression of the platein genes. (A) Products of the -platein RT-PCR experiments (see text), stained with ethidium bromide. Lane 1: RT-PCR products obtained using oligonucleotides AP11 and AP12 at low annealing stringency so that they bind to both genes; the two bands correspond in size to the expected fragments of the two genes, considering the deletion within the 2-platein gene. Lane 2: RT-PCR product obtained using oligonucleotide AP11 (common to both genes) and AP12 at a stringency condition specific for the 1-platein gene. Lane 3: RT-PCR product obtained as in lane 2 except for the use of oligonucleotide AP13, instead of AP12, at a stringency condition specific to the 2-platein gene. Lane M: molecular size markers (reported in base pairs on the left of the gel). (B) RT-PCR amplification product from total RNA obtained with primers designed from the ß/-platein gene sequence. M-MLV(+): RNA sample incubated with the reverse transcriptase enzyme. M-MLV(-): a control RNA sample treated in the same way but without reverse transcriptase. DNA size markers are shown to the left.