Fig. 4. Immunofluorescence localization of - and ß/-plateins in whole permeabilized cells. The dorsal surface of cells decorated with anti--platein (A and C) or anti-ß/-platein (B and D) are shown. Before staining, cells were permeabilized/extracted with either mild (0.25%; A and B) or stronger (1%; C and D) concentrations of Triton X-100. After mild permeabilization, plates are clearly visible after decoration with the -platein antibody (A), while appearing irregularly decorated with the anti-ß/-platein (B). However, after strong extraction, the plates are no longer detected with the anti--platein (C); many small vesicles appear just beneath the cell cortex, as seen in the center of this cell. With the same treatment, the plates are fully and strongly decorated with the anti-ß/-platein (D). Bars, 10 µm.