Fig. 6. CA125-C-TERM is transported to the cell surface of both CHO and HeLa cells as determined by FACS. CHO_MCAT-TAM2 (A) and HeLa_MCAT-TAM2 (B) cells, respectively, were transduced with retroviral particles encoding CA125-C-TERM. Following 3 days of incubation at 37°C, cells were dissociated from the culture plates using a protease-free buffer and processed with anti-CA125 antibodies (OC125). Primary antibodies were detected with anti-mouse antibodies coupled to Alexa-488. CA125 cell-surface localization was analyzed by FACS. Autofluorescence was determined with trypsin-treated cells (red curves). Non-transduced cells prepared in the absence of trypsin are indicated by green curves. CA125-C-TERM-transduced cells prepared in the absence of trypsin are indicated by blue curves.