Fig. 1. Control EGFP or HIF-1 hydroxylase. EGFP fusion proteins transiently expressed in subconfluent human U2OS osteosarcoma cells. Cells were transfected with pEGFP-N1 or with plasmids encoding PHD-EGFP or FIH-1-EGFP. Cells were fixed with cold 4% paraformaldehyde/PBS for 10 minutes. Left column, EGFP fluorescence microscopy; right column, fluorescence intensities visualised using a false colour table shading from black (lowest intensity), through blue, green, yellow, orange, red and purple to white (highest intensity).