Fig. 2. Three-dimensional two-photon confocal laser scanning microscopy of PHD1, PHD2, PHD3 and FIH-1. Different EGFP fluorescence intensities of single cells were visualised in false colours as indicated by the color table (right column). Therefore, up to 64 optical slices through the transfected cells were recovered by two-photon confocal laser scanning microscopy. After reconstruction of the optical slices, the distribution of the EGFP fluorescence within a single cell was visualised in three dimensions. A cut through the cell reveals the inside distribution. An overlay of all optical slices is shown in the inserts.