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Fig. 5. Effect of the transient overexpression of HIF-1{alpha} hydroxylases on HRE-luciferase expression. U2OS cells were cotransfected with the indicated HIF-1{alpha} hydroxylase construct and a hypoxia-inducible firefly luciferase gene. Luciferase values of three separate wells were divided by ß-galactosidase values to correct for variations of transfection efficiency and are given as means plus standard deviation.